American Medical Certification Association (AMCA) Phlebotomy Practice Exam 2026 - Free Phlebotomy Practice Questions and Study Guide

The quality of a blood sample is significantly influenced by the sample volume ratio, which refers to the proportion of blood collected relative to the anticoagulant present in the collection tube. Each tube is designed with a specific volume that must be filled to ensure the correct concentration of anticoagulant, which is critical for the accurate preservation of the blood sample and the validity of test results.

When the sample volume is inadequate, it can lead to an incorrect ratio of anticoagulant to blood, potentially causing clotting or the degradation of cellular components in the sample. This imbalance may result in inaccurate laboratory results, impacting diagnoses and treatment plans. Therefore, adhering to precise filling requirements by focusing on the sample volume ratio is essential for maintaining sample integrity and ensuring reliable laboratory analysis.

The other components listed, such as the mixing of anticoagulants, temperature storage, and time before processing, also play roles in the overall management of blood samples, but they do not directly determine the sample's quality concerning specific filling requirements.